Determining the extent to which a specimen is enlarged under a light microscope is a fundamental aspect of microscopy. This calculation relies on the objective lens magnification and the eyepiece lens magnification. Multiplying these two values yields the total enlargement factor. For example, an objective lens with a 40x power used in conjunction with an eyepiece lens of 10x power results in a 400x total magnification.
The ability to quantify the size of observed features is crucial for accurate scientific observation and documentation. Knowledge of the enlargement produced by the optical system facilitates precise measurement and comparison of microscopic structures. Historically, this calculation has been essential for advancements in fields such as biology, medicine, and materials science, enabling researchers to visualize and understand the intricacies of the microscopic world.