Determining the extent to which an object’s image is enlarged through a microscope is a fundamental procedure in microscopy. This determination relies on multiplying the magnifying power of the objective lens by the magnifying power of the eyepiece lens. For example, if an objective lens has a magnification of 40x and the eyepiece has a magnification of 10x, the overall magnification is 400x.
Accurate magnification determination is essential for proper interpretation of microscopic images and precise measurements of microscopic features. This process allows researchers to understand the true dimensions of cells, tissues, and other microscopic structures, facilitating advancements in biological research, medical diagnostics, and materials science. Historically, understanding image enlargement enabled early scientists to observe and describe microorganisms, laying the groundwork for germ theory and modern medicine.