Determining the overall enlargement achieved by a compound microscope involves a simple multiplicative process. This process combines the magnifying power of the objective lens and the eyepiece (ocular lens). The figure obtained by multiplying the objective lens magnification by the eyepiece magnification provides a readily understandable representation of the extent to which the observed specimen is enlarged. For instance, an objective lens with a 40x magnification, when used with an eyepiece having a 10x magnification, will yield a resultant amplification factor of 400x.
Understanding resultant amplification is essential for accurately interpreting microscopic observations. It allows researchers and students to assess the size and scale of microscopic structures, facilitating meaningful analysis and comparison of specimens. Historically, accurate measurement of magnification has been critical for advancements in fields like biology, medicine, and materials science, enabling the discovery and characterization of previously unseen details. Careful calculation promotes consistent data collection and reproducible experimental results, underpinning the scientific method.