The determination of the amount of oxygen required by microorganisms to decompose organic matter in a water sample is a crucial step in assessing water quality. This process involves measuring the dissolved oxygen content of a sample immediately, incubating a sealed sample at a specific temperature (typically 20C) for a set period (usually 5 days), and then measuring the dissolved oxygen again. The difference between the initial and final dissolved oxygen levels provides an indication of the biodegradable organic material present. For example, if a water sample initially contains 8 mg/L of dissolved oxygen, and after 5 days it contains 3 mg/L, then the calculated oxygen demand is 5 mg/L.
This evaluation is vital for monitoring the health of aquatic ecosystems and ensuring the effectiveness of wastewater treatment processes. Elevated levels indicate a higher concentration of organic pollutants, which can deplete oxygen levels in natural waters, harming aquatic life. Historically, the method has been a cornerstone of environmental monitoring, providing essential data for regulations and pollution control efforts, contributing to the maintenance of healthy water resources and protecting public health.