Biochemical oxygen demand, often abbreviated as BOD, represents the amount of dissolved oxygen microorganisms consume while decomposing organic matter within a water sample under aerobic conditions. It serves as a vital indicator of the organic pollution level in water bodies. The process of determining this value typically involves incubating a water sample at a specific temperature (usually 20C) for a defined duration (commonly 5 days). Oxygen levels are measured initially and after the incubation period; the difference quantifies the oxygen consumed during biodegradation. For example, if a water sample initially contains 8 mg/L of dissolved oxygen and, after five days of incubation, has 2 mg/L, the five-day BOD would be 6 mg/L.
Measuring oxygen consumption is critical for evaluating the effectiveness of wastewater treatment processes and for assessing the environmental impact of effluent discharge into rivers, lakes, and oceans. It informs regulatory bodies in setting discharge limits to protect aquatic ecosystems. Historically, the test has been a cornerstone of water quality monitoring, providing valuable data to track pollution trends and assess the health of aquatic environments.